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KMID : 1144820180240030213
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대한의생명과학회지
2018 Volume.24 No. 3 p.213 ~ p.220
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Upregulation of TNF-α by Triglycerides is Mediated by MEK1 Activation in Jurkat T Cells
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Lim Jae-Won
Yang Eun-Ju
Chang Jeong-Hyun
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Abstract
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Triglyceride (TG) is known to be associated with inflammatory disease including atherosclerosis. In a variety of atherosclerosis models, T lymphocytes are localized in the earliest lesions of atherosclerosis. T cell associated cytokines such as TNF-α and IFN-γ have pre-dominant inflammatory effects in chronic vascular diseases. In our previous study, we found that the expression of TNF-α and its receptor, TNF-αR was increased when Jurkat T lymphocyte cell lines were exposed to TGs. Therefore, experiments were conducted to determine which cell signaling pathway are involved in the increase of TNF-α and TNF-αR expression by TGs. To identify signal transduction pathways involved in TG-induced upregulation of TNF-α, we treated TG-exposed Jurkat T cells with specific inhibitors for MEK1, PI3K, NF-κB and PKC. We found that inhibition of the MEK1 pathway blocked TG-induced upregulation of TNF-α. However, the expression level of TNF-αR did not change with any signal transduction inhibitor. Based on this observation, we suggest that increase of exogenous TG induces increase of TNF-α expression through MEK1 pathway in Jurkat T cells. In addition, it was confirmed that the increase of TNF-α and TNF-αR expression by TGs occurs via different pathways.
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KEYWORD
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Triglyceride (TG), TNF-α, TNF-αR, MEK1, Jurkat T cells
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